BIOT 106 - Intro to Biotechnology Lab
PREREQUISITES/COREQUISITE: BIOT 100 - Survey of Biotechnology ;
and BIOL 105 - Biology I - Molecular and Cellular Processes
or Program Advisor Approval.
PROGRAM: Biotechnology
CREDIT HOURS MIN: 1
LAB HOURS MIN: 2
DATE OF LAST REVISION: Fall 2022
Students will learn the most basic laboratory techniques in biotechnology. Lab safety, documentation, units of measurement, instrumentation, lab solutions, aseptic technique, establishing a pure culture, plasmid DNA isolation, restriction enzyme digestion, DNA agarose gel electrophoresis, PCR, DNA ligation and transformation of bacterial cells will be introduced during the course.
MAJOR COURSE LEARNING OBJECTIVES: Upon successful completion of this course the student will be expected to: - Demonstrate appropriate documentation practices.
- Describe and apply safety rules.
- Learn and use various laboratory tools and measurements.
- Use and prepare solutions of varying concentration.
- Apply aseptic techniques.
- Learn separation methods and identify unknown molecules.
- Develop and use basic DNA manipulation techniques.
COURSE CONTENT: Topical areas of study include -
- Safety Rules and Record Keeping
- Metric Units- Weights and Measurements
- Concentration: pH and Buffers
- Centrifugation
- DNA Extraction
- Chromatography- Gel electrophoresis, Ion exchange, HPLC, affinity
- Protein assay
- Internet resources
Course Addendum - Syllabus (Click to expand)
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