| |
Aug 06, 2026
|
|
|
|
|
BIOT 227 - Genetic Engineering and DNA Analysis
PREREQUISITES: BIOT 201 - Cell Culture & Cell Processes or BIOT 211 - Analytic Methods in Biotechnology I
PROGRAM: Biotechnology
CREDIT HOURS MIN: 4
LECTURE HOURS MIN: 2
LAB HOURS MIN: 4
DATE OF LAST REVISION: Summer, 2011
Students will study the essential concepts and techniques in genetic engineering. Students will practice essential gene cloning procedures: isolation of DNA, restriction endonuclease digestion, agarose gel electrophoresis analysis, DNA ligation, and transformation into a host strain. Other essential techniques such as PCR, construction and screening of genomic or cDNA libraries, Southern and Northern blot analyses will be practiced. Students will understand the principles and ethical issues of animal or human cloning practices. Current methods for transfer and propagation of genes into plants and animals will be discussed. Various gene knockout techniques such as homologous gene recombination, site-directed mutagenesis, and RNAi will be introduced. The topics in genomics, proteomics, and bioinformatics will be discussed.
MAJOR COURSE LEARNING OBJECTIVES: Upon successful completion of this course the student will be expected to:
- Understand the structure of nucleic acid molecules.
- Fully understand the central dogma of biology: replication, transcription, and translation.
- Quantify the amounts of nucleic acids using spectrophotometric methods.
- Isolate DNA from various cells; bacteria, plants, and animals.
- Carry out restriction enzyme digestion.
- Carry out agarose gel electrophoresis.
- Comprehend the principles of PCR and practice its implications.
- Transform bacterial and eukaryotic cells with foreign DNA.
- Comprehend procedures for constructing genomic DNA or cDNA libraries.
- Comprehend procedures for screening libraries for identifying and cloning a specific gene.
- Comprehend nucleic acid hybridization techniques.
- Comprehend various mutagenesis methods and perform some of them.
- Comprehend the principles of reporter gene assays and be able to apply them in cloning procedures.
- Describe transformation and culture methods.
- Comprehend medical and forensic implications of molecular biology.
- Comprehend the importance of genomic projects.
COURSE CONTENT: Topical areas of study include -
- Basics recombinant DNA technology
- Small and large-scale isolation of DNA and RNA
- Restriction enzyme digestion
- Ligation and transformation of DNA
- Southern and Northern blot analysis
- PCR
- Construction and screening of DNA libraries
- Reporter gene screening methods
- Gel electrophoresis
- DNA sequencing analysis
Course Addendum - Syllabus (Click to expand)
|
|
