Sep 21, 2026  
2026-2027 Catalog 
    
2026-2027 Catalog

MEDL 226 - Microbiology Techniques II


PREREQUISITES: MEDL 101 - Fundamentals of Laboratory TechniquesMEDL 102 - Routine Analysis Techniques, MEDL 225 - Microbiology Techniques I, and Program Chair Approval
CREDIT HOURS MIN: 2 CREDIT HOURS MAX: 2
LECTURE HOURS MIN: 2 LECTURE HOURS MAX: 2
LAB HOURS MIN: 2 LAB HOURS MAX: 2
This course is a continuence of the principles of bacteriology including: gram negative and gram-positive bacilli and cocci, fastidious organisms and an overview of anaerobic organisms and acid-fast bacteria. Instruction in basic laboratory techniques in clinical bacteriology will also be included.

MAJOR COURSE LEARNING OBJECTIVES: MAJOR COURSE LEARNING OBJECTIVES: Upon successful completion of this course the student will be expected to:

  1. Identify and implement OSHA and Bloodborne Pathogen Standards, and critically analyze the implications of these policies on laboratory practices.
  2. Apply diagnostic criteria to identify and assess the acceptability of specimens, by understanding specimen characteristics and potential implications for testing outcomes.
  3. Demonstrate aseptic handling of bacteriologic specimens with a high level of proficiency.
  4. Analyze and differentiate bacterial, viral, and rickettsial specimens with an understanding of their descriptions and classifications.
  5. Demonstrate advanced decision-making skills in selecting appropriate media to utilize and process bacteriologic specimens, identify and detect microorganisms, and perform antimicrobial susceptibility testing.
  6. Employ advanced laboratory techniques, including biochemical, immunological, and serological methodologies, to detect and identify unknown organisms.
  7. Apply principles of quality assurance and quality control, demonstrating a comprehensive understanding of their impact on laboratory processes and outcomes, and integrating proactive strategies to optimize diagnostic accuracy.
  8. Demonstrate awareness of the necessity for corrective action in pre-analytical, analytical, and post-analytical situations, applying advanced problem-solving skills to address and rectify potential deviations from established protocols.
  9. Interpret and correlate test results with patient conditions, incorporating an understanding of the relationship between laboratory data and clinical outcomes.  , and drawing insightful connections between diagnostic findings and potential patient care implications.

 

 

COURSE CONTENT: Topical areas of study include -

  • Neisseria and Moraxella
  • Haemophilus and other fastidious gram negatives
  • Enterobacteriaceae
  • Vibrio, Aeromonas, Plesiomonas
  • Helicobacter and Campylobacter
  • NF GNB and miscellaneous GNR
  • Anaerobes
  • Aerobic gram-positive rods
  • M. tuberculosis/Mycobacteria
  • Antimicrobial susceptibility testing
  • Antibiotic mechanisms of action and resistance

Disease by Body System

  1. Respiratory infection
  2. Urinary tract
  3. GI infections/food poisoning
  4. CNS infections
  5. Skin/Soft tissue 

Laboratory Content:

  • Streaking isolation/interpretation
  • Gram staining
  • Identification of unknowns
  • Sensitivity testing (if applicable)
  • Case studies

Identification/differentiation testing (but not limited to)

  1. Coagulase
  2. Catalase
  3. Indole
  4. Novobiocin
  5. Oxidase
  6. Beta lactamase
  7. TSI slants

Laboratory Content:

  • Streaking isolation/interpretation
  • Gram staining
  • Identification of unknowns
  • Sensitivity testing (if applicable)
  • Case studies
  • Identification/differentiation testing (but not limited to)
  1. Coagulase
  2. Catalase
  3. Indole
  4. Novobiocin
  5. Oxidase
  6. Beta lactamase
  7. TSI slants