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May 03, 2024
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2022-2023 Catalog [ARCHIVED CATALOG]
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BIOT 101 - Introduction to Biotechnology
PREREQUISITES: BIOL 105 - Biology I - Molecular and Cellular Processes or BIOL 121 - General Biology I .
PROGRAM: Biotechnology
CREDIT HOURS MIN: 4
LECTURE HOURS MIN: 3
LAB HOURS MIN: 2
DATE OF LAST REVISION: Fall, 2015
Presents a basic overview of biotechnology emphasizing current DNA and RNA technologies and structure and function of biomolecules. The application of these techniques in the field of medicine, agriculture, forensics and environment is emphasized. Scientific methods, lab safety and regulations and ethics of the biotechnology industry will also be covered.
MAJOR COURSE LEARNING OBJECTIVES: Upon successful completion of this course the student will be expected to:
- Understand basic molecular genetics as it applies to organismal genomes.
- Describe processes of recombinant DNA technology and DNA cloning.
- Understand the importance of information technology in the field of bioinformatics.
- Discuss uses, purification and analysis of proteins as biotechnology products.
- Describe the uses of microorganisms as tools of biotechnology.
- Define the major mechanisms of plant transgenesis and discuss their applications.
- Review the use of transgenic and cloned animals in biotechnology research.
- Understand the processes of DNA fingerprinting and forensic analysis.
- Describe processes and products of medical biotechnology.
- Understand government regulations that guide the biotechnology industry.
- Discuss the ethics of using and manipulating living organisms for human benefit.
- Understand the impact of biotechnology on agriculture, food production, medical and environmental applications, and biofuels.
Upon successful completion of the lab student will be expected to:
- Demonstrate appropriate documentation practices.
- Describe and apply safety rules.
- Learn and use various laboratory tools and measurements.
- Use and prepare solutions of varying concentration.
- Apply aseptic techniques.
- Learn separation methods and identify unknown molecules.
- Develop and use basic DNA manipulation techniques.
COURSE CONTENT: Topical areas of study include - Lecture Course Content:
- Biotechnology overview
- Cellular Structure and Function
- Genes and genomes
- Mutations and Human Genetic disorders
- Recombinant DNA technology
- Proteins - Structure and Function
- Microbial biotechnology
- Plant biotechnology
- Animal biotechnology
- DNA forensics
- Bioremediation
- Medical and Pharmaceutical biotechnology
- Regulations and ethics
- Licensing and Patents
- Agricultural Applications
- Laboratory Course Content:
- Safety Rules and Record Keeping
- Metric Units- Weights and Measurements
- Concentration: pH and Buffers
- Centrifugation
- DNA Extraction
- Chromatography- Gel electrophoresis, Ion exchange, HPLC, affinity
- Protein assay
- Internet resources
Course Addendum - Syllabus (Click to expand)
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