May 04, 2024  
2022-2023 Catalog 
    
2022-2023 Catalog [ARCHIVED CATALOG]

BIOT 222 - Microbiology Laboratory


PREREQUISITES: BIOL 121 - General Biology I  or BIOL 105 - Biology I - Molecular and Cellular Processes  and CHEM 105 - General Chemistry I .
PREREQUISITES/COREQUISITE: BIOT 221 - Microbiology  

PROGRAM: Biotechnology
CREDIT HOURS MIN: 2
LAB HOURS MIN: 4
DATE OF LAST REVISION: Fall, 2017

A conventional laboratory of exercises, demonstrations and discussions. Laboratory exercises are designed to enable students to achieve proficiency in the principles and techniques necessary for cultivation of microorganisms using aseptic techniques and for performing and interpreting biochemical tests. The laboratory exercises will be filled out weekly and turned in to be graded.

MAJOR COURSE LEARNING OBJECTIVES: Upon successful completion of this course the student will be expected to:

  1. Understand Bright-field Microscopy.
  2. Follow laboratory safety procedures.
  3. Carry out different staining methods and identify different groups of microorganisms.
  4. Perform aseptic technique.
  5. Prepare various microbiological culture media and sterilize them.
  6. Understand and recognize various bacterial morphologies.
  7. Comprehend the use of physical and chemical methods and antimicrobial agents in the control of microorganisms.
  8. Understand the fundamentals of recombinant DNA technologies, genetic engineering and biotechnology as they relate to microbiology, medicine, industry, and the environment.
  9. Understand the general principles of bacterial metabolism, nutrition, reproduction, and energy liberation and storage.


COURSE CONTENT: Topical areas of study include -  

  • Microscopy and staining
  • Growth and metabolism of bacteria
  • Sterilization and disinfection
  • Bacteria
  • Characteristics of prokaryotic and eukaryotic cells
  • Biotechnology; Recombinant DNA
  • Eukaryotic microorganisms
  • Microbial interactions
  • Role of DNA in cell
  • Bacterial genetics
  • Antimicrobials
  • Classification of microorganisms

 
Course Addendum - Syllabus (Click to expand)