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May 05, 2024
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2022-2023 Catalog [ARCHIVED CATALOG]
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BIOT 201 - Cell Culture and Cellular Processes
PREREQUISITES: BIOT 101 - Introduction to Biotechnology or BIOT 100 - Survey of Biotechnology and BIOT 106 - Introduction to Biotechnology Laboratory ; and CHEM 105 - General Chemistry I or CHEM 111 - Chemistry I or Program Advisor Approval.
PROGRAM: Biotechnology
CREDIT HOURS MIN: 4
LECTURE HOURS MIN: 2
LAB HOURS MIN: 4
DATE OF LAST REVISION: Fall, 2019
The student will be introduced to major biochemical pathways, cellular structure and function at a molecular level. Topics to be considered include the structure and function of the cell membrane, cytoskeleton and various organelles. Cellular respiration will be discussed. Protein synthesis, processing and export will be examined. Those processes involved in cell division will also be investigated and related to cancer. The laboratory will center upon techniques involving animal, plant, fungi and bacterial cell cultures. Students will be taught how to isolate, culture and preserve prokaryotic organisms. Students will be taught how to maintain and preserve eukaryotic cell cultures. Students will learn to procure cell cultures from ATCC and other repositories.
MAJOR COURSE LEARNING OBJECTIVES: Upon successful completion of this course the student will be expected to:
- Know the structure and function of major cell components.
- Understand the molecular basis of cell division, signal transduction, and gene expression.
- Be familiar with the concepts of metabolism and bioenergetics within the cell.
- Know and practice safety measures and aseptic techniques in the cell culture laboratory.
- Identify and properly use basic equipment necessary for cell culture.
- Be able to make up the medium and understand the rationale for its composition.
- Be familiar with the practices and principles of cell line procurement, initiation, maintenance and preservation.
- Be familiar with methods of cell counting and passaging.
- Detect and recognize contamination of cell cultures, and its potential source(s).
- Understand the technologies behind the various ways to visualize, sort, and count cells.
- Be familiar with various methods of transfection/transformation of prokaryotic and eukaryotic cells.
- Recognize ways by which cell culture can give rise to products/activities of economic importance, or play a role in biotechnology research.
- Understand the role good documentation plays in all aspects of cell culture (including history of cells, nature of cell substrate, and genetic stability) as it applies to validation in cGMPs.
- Understand the concepts of Master Cell Bank (MCB) and Working Cell Bank (WCB) and their importance in the preservation of purity and integrity of cell lines as observed in cGMPs.
- Understand how to assemble, adjust and maintain a light microscope.
- Be familiar with and capable of utilizing the various types of illumination that are commonly used with a light microscope.
COURSE CONTENT: Topical areas of study include -
- Basic cell biology (structure, function, biochemistry)
- Molecular biology of the cell (including genetics, gene expression, signal transduction)
- Microscopy of the cell (SEM, TEM, AFM, light and confocal microscopy)
- Flow cytometry and FACS
- Aseptic technique and safety in the lab
- Media composition and preparation
- Incubators/growth chambers for cell culture
- Use of laminar flow hoods
- Cell culture initiation, maintenance, and preservation
- Cell culture record keeping
- Cell transformation/transfection
- Scale-up of cell cultures for manufacturing purpose
- Cell culture to transgenic organisms: creation and uses
- Use of cell culture in research
- Observation of cGMPs with respect to cell culture.
GRADING POLICY
| A |
90-100 |
| B |
80-89 |
| C |
70-79 |
| D |
60-69 |
| F |
0-59 |
Course Addendum - Syllabus (Click to expand)
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