| |
May 04, 2024
|
|
|
|
|
2022-2023 Catalog [ARCHIVED CATALOG]
|
BIOT 211 - Analytic Methods in Biotechnology I
PREREQUISITES: BIOT 101 - Introduction to Biotechnology or BIOT 100 - Survey of Biotechnology and BIOT 106 - Introduction to Biotechnology Laboratory ; and CHEM 105 - General Chemistry I or CHEM 111 - Chemistry I ; or Program Advisor Approval.
PROGRAM: Biotechnology
CREDIT HOURS MIN: 3
LECTURE HOURS MIN: 2
LAB HOURS MIN: 2
DATE OF LAST REVISION: Fall, 2019
Students will become familiar with the theory and application of many analytical methods currently utilized in the field of biotechnology. These methods will include: ELISA and immunoaffinity techniques; methods for determining enzymatic activity; spectrophotometric methods; chromatographic methods; electrophoresis; light and electron microscopy. When feasible, techniques will be practiced in the laboratory setting. Methods utilizing radioactive isotopes will be discussed. Considerable emphasis will be placed on proper methods for data recording, analysis and presentation.
MAJOR COURSE LEARNING OBJECTIVES: Upon successful completion of this course the student will be expected to:
- Understand and be proficient in the data collection, presentation and basic statistical analysis of experimental data.
- Understand the basic physical characteristics of liquids and solids including temperature, pH, conductivity, weight and density of liquids, solids and gasses and should be proficient in the measurement of these characteristics.
- Comprehend the theoretical basis for spectrophotometry and how spectrophotometry can be used in analysis and monitoring of research and industrial processes.
- Be proficient in the use of spectrophotometry in the measurement of cell density, DNA and RNA concentration and enzyme activity.
- Be able to operate microfuges and centrifuges properly and safely.
- Be able to utilize publicly accessed DNA databases and DNA analysis software.
- Understand the function and applications of restriction endonucleases in the analysis and manipulation of DNA.
- Understand the theoretical basis of blotting and hybridization probing.
- Be able to perform a Southern blot and interpret the results.
- Comprehend the theoretical basis of PCR and rationale used in primer selection.
- Understand the various applications of PCR in research and industry.
- Understand dideoxy nucleotide sequencing and be able to interpret a sequencing gel.
- Comprehend the rationale for using various types of bioassays.
- Be able to carry out and interpret the results of simple bioassays.
COURSE CONTENT: Topical areas of study include -
- Data Collection
- Measurement of physical characteristics
- Centrifugation
- Spectrophotometry
- Southern Blotting
- Protein and DNA electrophoresis
- Hybridization probes
- DNA databases
- RT-PCR
- Restriction endonucleases
- DNA sequencing
- Polymerase chain reaction
- Bioassays
- Gradient centrifugation
- Ultra centrifugation
GRADING POLICY
| A |
90-100 |
| B |
80-89 |
| C |
70-79 |
| D |
60-69 |
| F |
0-59 |
Course Addendum - Syllabus (Click to expand)
|
|
