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May 05, 2024
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2022-2023 Catalog [ARCHIVED CATALOG]
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BIOT 212 - Analytic Methods in Biotechnology II
PREREQUISITES: BIOT 211 - Analytic Methods in Biotechnology I
PROGRAM: Biotechnology
CREDIT HOURS MIN: 3
LECTURE HOURS MIN: 2
LAB HOURS MIN: 2
DATE OF LAST REVISION: Summer, 2011
Students will continue their study of the theory and application of many analytical methods currently utilized in the field of biotechnology. These methods will include: centrifugation, light and electron microscopy, restriction endonuclease digestion, agar and acrylamide electrophoresis of nucleic acids, Southern and Northern blotting, polymerase chain reaction and bioassays. When feasible, techniques will be practiced in the laboratory setting. Methods utilizing radioactive isotopes will be discussed. Considerable emphasis will be placed on proper methods for data recording, analysis and presentation.
MAJOR COURSE LEARNING OBJECTIVES: Upon successful completion of this course the student will be expected to:
- Understand the chemical characteristics of amino acids and how they impact protein structure.
- Be able to conduct basic flirtation methods and describe the theory underlying TFF.
- Be able to analyze proteins using spectrophotometric and PAGE analysis.
- Demonstrate an understanding of ion exchange, affinity, size exclusion and reverse phase chromatography and their application in HPLC and LC.
- Understand the structure of antibodies and the basis of antibody specificity.
- Understand how hybridomas are generated and the uses of monoclonal antibodies.
- Demonstrate an understanding of the applications of antibodies in research and industry including immunoprecipitation, ELISA, immunoaffinity chromatography and Western blotting.
COURSE CONTENT: Topical areas of study include -
- HPLC
- Chromatography
- Protein Assay
- Antibody structure and specificity
- Protein Kinetics
- Immunoprecipitation
- Protein Purification
- Immunoaffinity chromatography
- ELISA
- Western blotting
- Monoclonal antibodies
- Policy for possible alternatives.
Course Addendum - Syllabus (Click to expand)
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