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May 04, 2024
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2022-2023 Catalog [ARCHIVED CATALOG]
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BIOT 235 - Biotechnology Laboratory
PREREQUISITES: BIOL 107 - Biology II - Diversity of Life and CHEM 105 - General Chemistry I
PREREQUISITES/COREQUISITE: BIOL 221 - Molecular Biology
CREDIT HOURS MIN: 3
LECTURE HOURS MIN: 2
LAB HOURS MIN: 2
DATE OF LAST REVISION: Summer, 2011
Presents an in-depth overview of basic biotechnology laboratory skills emphasizing chromatography techniques, methods of DNA and protein electrophoresis, processes of immunoassays, data management skills, recombinant DNA technology, and the polymerase chain reaction.
MAJOR COURSE LEARNING OBJECTIVES: Upon successful completion of this course the student will be expected to
- Graph data, create standard curves.
- Isolate DNA from cell extracts.
- Separate biochemicals using chromatographic techniques.
- Separate DNA and proteins using electrophoresis.
- Construct recombinant DNA using common cloning vectors.
- Isolate recombinant proteins from a DNA library.
- Amplify segments of DNA using the polymerase chain reaction.
- Understand the process of DNA sequencing.
- Utilize bioinformatics databases for genome and proteome analysis.
- Understand the use of restriction enzymes.
- Use spectrophotometry to determine protein concentrations.
- Perform immunoassays using monoclonal antibodies.
COURSE CONTENT: Topical areas of study include -
- ELISA assays
- Bacterial transformation
- Chromatography techniques
- Protein fingerprinting
- Isolation of DNA
- Restriction digests
- PCR
- DNA fingerprinting
- HPLC
- GC
- DNA sequencing
- Genomics
- Bioinformatics
Course Addendum - Syllabus (Click to expand)
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