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May 04, 2024
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2022-2023 Catalog [ARCHIVED CATALOG]
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BIOT 237 - Plant Tissue Culture
PREREQUISITES: BIOT 201 - Cell Culture and Cellular Processes and CHEM 105 - General Chemistry I .
PROGRAM: Biotechnology
CREDIT HOURS MIN: 4
LECTURE HOURS MIN: 2
LAB HOURS MIN: 4
DATE OF LAST REVISION: Fall, 2017
The student will be introduced to basic techniques of plant tissue culture. This is the aseptic culture of plant cells, tissues, organs and plants. This course seeds to familiarize students with the basic principles of tissue culture and to expose them to their many applications. The course includes media preparation, isolation of explants, and establishment of calluses from suspension cultures, growth factor bioassays, and regeneration of whole plants from tissue and plant and genetic engineering techniques. We will also discuss the theory, production and societal implications of transgenic plants.
MAJOR COURSE LEARNING OBJECTIVES: Upon successful completion of this course the student will be expected to:
- Know the methods of plant tissue culture.
- Understand the characteristics of plant life cycle and the characteristics of different plant classes.
- Know and practice safety measures and aseptic techniques in the plant tissue culture laboratory.
- Identify and properly use basic equipment necessary for plant tissue culture.
- Be able to make up the medium and understand the rationale for its composition.
- Be familiar with the practices and principles of plant propagation.
- Be familiar with methods of cell counting.
- Detect and recognize contamination of plant tissue cultures, and its potential source(s).
- Be familiar with various methods of transfection/transformation of plant cells.
- Be familiar with various methods of DNA extraction from plant tissue.
- Recognize ways by which plant tissue culture can give rise to products/activities of economic importance, or play a role in biotechnology research.
- Understand the role good documentation plays in all aspects of plant tissue culture.
COURSE CONTENT: Topical areas of study include -
- Plant tissue culture methodologies
- Plant life cycles
- Characteristics of plant classes
- Lab safety
- Aseptic techniques
- Basic lab equipment used in plant tissue culture
- Media preparation
- Understand media components
- Plant propagation
- Cell counting methods
- Culture contamination types and sources
- Transfection/transformation methods
- Plant DNA extraction methods
- Products of plant tissue culture
- Role of plant tissue culture
- Good documentation practices
Course Addendum - Syllabus (Click to expand)
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