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May 04, 2024
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2022-2023 Catalog [ARCHIVED CATALOG]
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BIOT 241 - Immunology and Immunological Processes
PREREQUISITES: BIOT 211 - Analytic Methods in Biotechnology I
PROGRAM: Biotechnology
CREDIT HOURS MIN: 4
LECTURE HOURS MIN: 2
LAB HOURS MIN: 4
DATE OF LAST REVISION: Summer, 2011
Students will get a brief survey of the components of the immune system and how they interact. The topics covered will include, B and T cell development, activation and culture, the role of cytokines, their production and purification, signal transduction processes in B-cell activation, the role of MHC complexes, immunoglobulin synthesis and origins of diversity, antigen-antibody interactions, practical aspects of raising and purifying polyclonal and monoclonal antibodies, handling and labeling of antibodies, applications of antibodies including Western blotting, ELISA, and immunohistochemistry.
MAJOR COURSE LEARNING OBJECTIVES: Upon successful completion of this course the student will be expected to:
- Describe the components of the immune system and the role of each.
- Describe the role of antibodies and cytokines, how they function and how they are produced and how they might be manufactured.
- State what constitutes an effective immunogen, how to produce a vaccine and how to isolate and utilize antibodies.
- Purify antibodies by chromatography, perform a Western blot and an ELISA.
- Successfully perform all of the calculations required for the laboratory.
COURSE CONTENT: Topical areas of study include -
- Overview of the Immune Response/System
- The Innate Immune System
- Cytokines
- Overview of cells and tissues of the specific immune system
- Antigens and immunogens
- Antigen Processing and MHC Complexes
- Vaccines and vaccine production
- Antibody structure and function
- Antibody diversity
- Antigen-antibody interactions
- Antibody applications
- Production and purification of antibodies
- Production and purification of cytokines
- B-cell development and biology
- T-cell development and biology
- Laboratory:
- Antigen preparation
- SDS-PAGE Electrophoresis of whole cell homogenate and antigen recovery
- Immunogen preparation with adjuvant
- Isolation and storage of serum from blood
- Estimation of serum protein
- Purification of IgG from serum
- Isotyping of serum and purified antibody
- Labeling and QC of purified antibody
- Western blot
- ELISA
- Immunoprecipitation and immunoaffinity chromatography
Course Addendum - Syllabus (Click to expand)
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