2022-2023 Catalog [ARCHIVED CATALOG]
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MEDL 216 - Microbiology Techniques
PREREQUISITES: MEDL 101 - Fundamentals of Laboratory Techniques , MEDL 102 - Routine Analysis Techniques and Program Chair Approval
PROGRAM: Medical Laboratory Technology
CREDIT HOURS MIN: 4
LECTURE HOURS MIN: 2
LAB HOURS MIN: 4
DATE OF LAST REVISION: N/A
This course will instruct the student in the principles of bacteriology including: gram negative and gram-positive bacilli and cocci, fastidious organisms and an overview of anaerobic organisms and acid-fast bacteria. Instruction in basic laboratory techniques in clinical bacteriology will also be included.
MAJOR COURSE LEARNING OBJECTIVES: Upon successful completion of this course the student will be expected to:
- Comply with all institutional safety policies, OSHA, and Bloodborne Pathogen Standards.
- Use established criteria to identify and evaluate specimen acceptability.
- Demonstrate proficiency in aseptic handling of bacteriologic specimens.
- Demonstrate an understanding of bacterial, viral and rickettsial descriptions and classifications.
- Select the proper media for and correctly process bacteriologic specimens in accordance to identification and detection of microorganisms and antimicrobial susceptibility testing.
- Identify unknown organisms using techniques presented in laboratory exercises in reference to biochemical, immunological, serological methodologies for identification and detection of microorganisms.
- Apply principles of quality assurance and quality control.
- Recognize the need for and implement corrective action as applicable for pre-analytical, analytical, and post-analytical situations.
- Correlate test results with patient conditions.
COURSE CONTENT: Topical areas of study include -
LECTURE CONTENT
- Bacterial cell structure, physiology, metabolism, and genetics
- Host-pathogen interaction
- Specimen collection and handling
- Colony morphology/microscopic examination
- Staphylococci
- Streptococci
- Neisseria and Moraxella
- Haemophilus and other fastidious gram negatives
- Enterobacteriaceae
- Vibrio, Aeromonas, Plesiomonas
- Helicobacter and Campylobacter
- NF GNB and miscellaneous GNR
- Anaerobes
- Aerobic gram-positive rods
Disease by Body System
- Respiratory infection
- Urinary tract
- GI infections/food poisoning
- CNS infections
- Skin/Soft tissue
M. tuberculosis/Mycobacteria
Antimicrobial susceptibility testing
Antibiotic mechanisms of action and resistance
Laboratory Content:
- Streaking isolation/interpretation
- Gram staining
- Identification of unknowns
- Sensitivity testing (if applicable)
- Case studies
Identification/differentiation testing (but not limited to)
- Coagulase
- Catalase
- Indole
- Novobiocin
- Oxidase
- Beta lactamase
- TSI slants
Course Addendum - Syllabus (Click to expand)
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